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YMC Carotenoid HPLC Column, C30, 3 µm, 100 mm x 4.6 mm

YMC Carotenoid HPLC Column, C30, 3 µm, 100 mm x 4.6 mm

YMC Carotenoid, C30, Column, proprietary, 3 µm, 100 x 4.6 mm
Artikelnummer: CT99S03-1046WT
Antal/fp: 1

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Produktbeskrivning Teknisk specifikation Dokument

- C30 chains
- very lipophilic
- exceptional selectivity pattern
- isomer recognition
- polar carotenes
- polar and nonpolar xanthophylls
- steroids
- retinols
- fat-soluble vitamins
- LC-MS applications

General
The separation of geometric and positional isomers is a challenging task in reversed phase chromatography. Subtle molecular differences have to be recognized and resolved by this particular stationary phase. Sander et al. have conclusively shown that polymeric C30 HPLC stationary phases are able to discriminate isomeric structures of long chain molecules.

Properties
Compared to classical C18 stationary phases, YMC Carotenoid is much more hydrophobic. Even when pure organic eluents are applied, many sample solutes are retained. The use of non-aqueous reversed phase mobile phases facilitates 100% solvent recycling and LC-MS applications. The YMC Carotenoid stationary phase provides sufficient phase thickness to enhance interaction with long chained molecules (see figure on right). Therefore, geometric and positional isomers of conjugated double bonding systems are recognised and resolved by the YMC Carotenoid phase. The resolving power of YMC Carotenoid for isomers can be verified by the separation of carotenoids, which has been subject of considerable research efforts in the past. Carotenoids are found in a variety of natural sources including fruits and vegetables. In addition, carotenoids are considered as potential drugs for cancer intervention or prevention. Despite the complexity of carotenoid extracts and the minor shape differences between carotenoid isomers, the separation, identification and quantification of these compounds can be achieved by using YMC Carotenoid columns.

Applications
YMC Carotenoid columns are successfully used in the food industry, for the analysis of vitamin formulations, in environmental analysis, and for the control of algal growth. Other potential applications include the separation of prostaglandins and leucotrienes.